FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2591_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2591_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3473422
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC24600.039.176273
GAGCAAA83500.038.751341
AGCGAAA26200.036.8288881
GCGAAAG26250.036.731742
GAGCGAA19900.034.634491
CAAAAGC104150.030.054694
GCAAAAG113650.027.5436083
AAAGCAG153050.024.9977476
AGCAAAA127900.024.6568662
AAGCAGG149400.024.44487
GTGATCG5750.022.6855189
AAAAGCA151150.020.9491945
TATATCG2506.0058526E-520.28865
GTTACGT4652.0372681E-1020.2828351
GTATTAT36600.020.0206681
ACTCCGA7100.019.4148271
GTATATA20500.018.4029641
ACATGGT22850.017.4409645
TAGTACC16650.017.4073854
TACTATA13700.016.933572
Produced by FastQC (version 0.11.5)