FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2591_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2591_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544833
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6050.068.348611
GAGCAAA51800.049.1572531
AGCGAAA12450.039.623221
GCGAAAG12400.039.1717452
CAAAAGC66200.038.743644
CGAAAGC13850.035.5745933
GCAAAAG72050.034.694923
CCGACTA4150.031.4253144
TATATCG2202.2815584E-829.6397845
AAAGCAG110500.026.882946
CACCGAC5100.025.5871642
GCCGACC2301.1067241E-625.210882145
AGCAAAA99150.025.0796362
AAAAGCA104400.024.706135
CGTATGC2101.5634487E-524.1523598
AAGCAGG126700.023.7325137
TTATCGC1550.00180969423.3861182
CCGACGC3503.5179255E-922.779833145
CTAGTAC3554.1618478E-922.4514883
GACGACT2603.2255666E-622.293174
Produced by FastQC (version 0.11.5)