FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2591_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2591_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975358
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG30890.10381943954307347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9100.058.997351
GAGCAAA68750.049.9149441
ACATGGT27450.038.014025
AGCGAAA17550.037.2055321
CGAAAGC19550.034.8415453
CGTCGGT850.002745648634.110554145
GCGAAAG20100.033.9007032
GCTATAT7750.032.764871
GGTCCTA41700.031.4559369
CAAAAGC114850.030.157734
AAGCAGG132750.030.1325237
GACATGG35700.028.6188774
GCAAAAG122800.027.9702433
GCTACTA8300.027.940369
GTTGACA36750.027.8358141
AAAGCAG144000.027.8287416
GCATGTT39700.027.5605684
TAGCATG40000.027.5461252
GTCACGT5400.026.8706631
TAGTCGG5200.026.4790928
Produced by FastQC (version 0.11.5)