FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2590_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2590_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4809799
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCT237000.4927440834845698No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC214040.44500820096640215No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA196030.4075638087994945No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA160820.33435908652315827No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC154150.3204915631609554No Hit
AGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTCAATCC138860.2887022929648412No Hit
AAAGAGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAAT138670.28830726606246954No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG138110.2871429762449533No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG136180.2831303345524418No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT135720.2821739536309106No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG135010.2806978004694167No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC112750.23441728022314448No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA103630.21545598890930787No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG101330.2106740843016517No Hit
CATTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA97240.20217061045586313No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGG96950.2015676746575065No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG96870.20140134754071842No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA92430.19217019255898218No Hit
GTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGCA91160.18952974957997204No Hit
GCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTC87420.18175395687013118No Hit
GAAAGAGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAA84950.17661860713930042No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT83180.17293861968036503No Hit
GTGCTTTAACAGTGCATTTGGATCGTCAAGTTCTACTACTATTGATTCGC79550.1653915267561077No Hit
GAACAATAAGGAGCAGTTTTCTTGACTCTGCTGAAAATCCTTCCAATTGT76470.15898793275976814No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA76380.1588008147533816No Hit
GTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTC76270.15857211496779802No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA75890.15778206116305485No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC74960.15584850843039386No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC71680.1490290966420842No Hit
GGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGC70420.14640944455267257No Hit
CATTAGCCTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAAT69880.1452867365143533No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG68860.1431660657753058No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT68270.141939403288994No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG65730.1366585173309737No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT59820.12437110157825723No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC57650.11985947853538162No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA57500.11954761519140404No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG57360.11925654273702496No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCG56180.11680321776440139No Hit
GACTTATTTCAAATCTGTGCTTTAACAGTGCATTTGGATCGTCAAGTTCT56020.11647056353082531No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT55590.11557655527808959No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG55220.1148072923629449No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG54430.11316481208466299No Hit
GTTTTCTTGACTCTGCTGAAAATCCTTCCAATTGTGGTGATGCATACAGG54090.11245792183831382No Hit
AGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGC52430.10900663416496198No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT49970.10389207532372974No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT49790.10351783931095665No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG49040.10195852259106877No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC48820.10150112301990166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA15600.0104.651021
GCAAAAG176300.072.41363
CAAAAGC178950.071.457524
AAGCAGG219100.065.906997
AGCGAAA28100.063.520561
AGCAGGT188550.062.0620428
AGCAAAA214800.060.0672072
AGGCTCG3300.057.108078145
CGAAAGC33450.055.2345243
GCAGGTA137000.054.9537859
GCAGGTC76450.054.7839139
CAAGCTA12700.054.7649154
GCGAAAG34200.053.4104352
AAGCTAA14100.049.3282935
GCTAAAT14350.048.4714367
GAGCAAA272450.048.0968361
AAAGCAG309350.046.935416
ACTATTC36450.046.5155758
TTTAAAC36500.046.450413
AAGGGTG28400.046.4298255
Produced by FastQC (version 0.11.5)