FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2589_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2589_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3826132
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT38500.10062381538326434No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7900.058.770461
GGTACGA400.006058074554.361642
AGCGAAA14950.046.0986981
GAGCAAA76600.043.470051
GCGAAAG17550.039.235372
CGAAAGC17950.039.151753
TGACGCG1751.0131225E-733.13298145
CAAAAGC98800.032.9958844
TTACGTG6600.031.8482342
TATATCG3650.029.772025
GCAAAAG112700.028.4789353
GTTACGT8300.027.0950411
TACGTGT7950.026.428643
GTATTAT51700.023.9945051
AAAGCAG156000.023.9626566
ATCGTGG5550.023.4960618
TATCGTG5450.022.5978957
AAGCAGG166500.022.3212597
AAAAGCA144650.022.2369085
TAGTACC15850.021.4833099
Produced by FastQC (version 0.11.5)