FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2589_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2589_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3808581
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10950.058.9605561
GAGCAAA89000.044.5844081
AGCGAAA26550.039.3444671
CGAAAGC28000.036.487553
GCGAAAG29300.035.6222272
CAAAAGC128250.031.1270474
ACATGGT29650.029.078865
GCAAAAG136200.028.7276613
AAGCAGG168900.027.3692747
ACTCCGA7500.027.0821061
AAAGCAG172500.026.6713776
GTCACGT9900.026.3786771
AGACGAC5000.026.0847173
GCGATTA2259.135092E-725.772497145
AGCAAAA156100.024.9343222
GTTACGT4800.024.1804521
TAGTCGG4650.023.373718
GTCGGCT10650.023.1307726
GTACGGT1902.1862803E-422.9077971
GGTTATA8250.022.8615191
Produced by FastQC (version 0.11.5)