FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2589_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2589_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175607
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA238941.0982682074473928No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC196240.902001142669609No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG193140.8877522456951094No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG134360.6175747733850828No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGG98290.45178196245921254No Hit
TCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTCATCTGGGTCTTCAA87020.3999803273293384No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA86600.3980498316102127No Hit
ATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTC85350.39230430863662413No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG82890.38099711942460196No Hit
GAGCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCT81900.37644666522951986No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT79510.3654612253040186No Hit
GCTTAATGCTGGTCCGTCTTCTGTCTTCTTTACCTATAATGAGGAACCCT75380.34647801739928213No Hit
GAATAAAAGAACTTCGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA75200.34565066209108536No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCG73980.340043031668863No Hit
CCTATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGATGTGCT72090.33135580093279715No Hit
GTTCATTGATGCTTAATGCTGGTCCGTCTTCTGTCTTCTTTACCTATAAT72050.33117194419764234No Hit
TAACAAGACCACTAAAAGGCTAACAATTCTTGGAAAAGATGCCGGCACTT62340.2865407217388067No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC60970.28024362855975365No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT60610.27858891794336016No Hit
ATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCAGTC54100.24866623429691115No Hit
CTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAATGCTGGTC53140.24425367265319517No Hit
GTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTC51860.23837025712824053No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC51680.23754290182004378No Hit
AGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTCAATCC51640.23735904508488895No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG51150.23510680007924226No Hit
CTAAAAGGCTAACAATTCTTGGAAAAGATGCCGGCACTTTAATTGAAGAC50760.23331419691148264No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG48860.2245810019916281No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC48170.22140947331020724No Hit
CATTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA47910.2202144045317008No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG47690.21920319248834924No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC47290.21736462513680088No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA46330.2129520634930849No Hit
ATGCTGGTCCGTCTTCTGTCTTCTTTACCTATAATGAGGAACCCTCTCAG45620.20968860644408663No Hit
CAATTAAAGTGCCGGCATCTTTTCCAAGAATTGTTAGCCTTTTAGTGGTC42810.19677267079945965No Hit
TTTTTGGACAGTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAA42350.19465831834517908No Hit
GGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATG42170.19383096303698233No Hit
AGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTCTTCTGTCTTCTT42050.19327939283151782No Hit
GTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAATGGA39800.1829374514790585No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT38380.17641053738106194No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC38030.17480179094845713No Hit
GCATTAAGCATCAATGAACTGAGTAACCTTGCTAAGGGGGAAAAGAATGT38020.17475582676466844No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT38000.174663898397091No Hit
GTCCTGAGAGGGTTCCTCATTATAGGTAAAGAAGACAGAAGACGGACCAG37550.17259551012659913No Hit
CTTTAATTGAAGACCCAGATGAAAGCACATCCGGAGTGGAGTCCGCCGTC36200.16639034531512356No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG36110.16597666766102517No Hit
AGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG35980.16537913327177195No Hit
GCTTTCATCTGGGTCTTCAATTAAAGTGCCGGCATCTTTTCCAAGAATTG35850.16478159888251878No Hit
GTCTTGTTATCATCCATTTCATCCTAAGTGACGGGTTCTTTTCCTGTCTC34950.16064482234153504No Hit
GTCTCGCACTCGCGAGATACTGACAAAAACCACAGTGGACCATATGGCCA34880.1603230730550141No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA34590.15899011172514152No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA34340.15784100713042382No Hit
CATCTGGGTCTTCAATTAAAGTGCCGGCATCTTTTCCAAGAATTGTTAGC34280.15756522202769158No Hit
GCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTC34110.15678383090328354No Hit
ACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG34020.15637015324918516No Hit
TAGCAAGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTCTTCTGTC33580.15434772916248202No Hit
CATTATAGGTAAAGAAGACAGAAGACGGACCAGCATTAAGCATCAATGAA33430.1536582664056514No Hit
TTCTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAATGCTGG32670.15016498843770956No Hit
CCTCATTATAGGTAAAGAAGACAGAAGACGGACCAGCATTAAGCATCAAT31880.14653381791840162No Hit
GTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGCA30440.13991497545282763No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG30360.139547261982518No Hit
GAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAAT29980.13780062299854706No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC29950.13766273044718094No Hit
GGACCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGA29760.13678941095519548No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA29180.13412348829545043No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA28500.13099792379781827No Hit
GATGAAAGCACATCCGGAGTGGAGTCCGCCGTCCTGAGAGGGTTCCTCAT28490.13095195961402956No Hit
GCCTTTTAGTGGTCTTGTTATCATCCATTTCATCCTAAGTGACGGGTTCT28400.1305382819599312No Hit
GATGATAACAAGACCACTAAAAGGCTAACAATTCTTGGAAAAGATGCCGG27770.12764253838124257No Hit
TCAATGAACTGAGTAACCTTGCTAAGGGGGAAAAGAATGTGCTAATTGGG27460.12621764868379262No Hit
GAGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC27390.12589589939727167No Hit
GCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCAGTCTCGCACT26630.12240262142932984No Hit
CTTCTTTACCTATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCG26570.12212683632659758No Hit
GAAAAGAACCCGTCACTTAGGATGAAATGGATGATAACAAGACCACTAAA25950.1192770569316977No Hit
GTGGACCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAA25730.1182658448883461No Hit
GGATGAAATGGATGATAACAAGACCACTAAAAGGCTAACAATTCTTGGAA25610.11771427468288159No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATC25310.11633534916922036No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA24780.11389924742841882No Hit
CTCCGGATGTGCTTTCATCTGGGTCTTCAATTAAAGTGCCGGCATCTTTT24700.11353153395810915No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA23950.11008422017395605No Hit
ATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGT23860.10967054251985768No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT23430.10769408261694323No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT23400.1075561900655771No Hit
CATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAATGGATGATA23270.1069586556763239No Hit
TCTTTACCTATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGA22340.10268398658397403No Hit
ATACTGACAAAAACCACAGTGGACCATATGGCCATAATTAAGAAGTACAC22250.10227030892987565No Hit
ACAGTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGC21930.100799455048637No Hit
GTCTTCTGTCTTCTTTACCTATAATGAGGAACCCTCTCAGGACGGCGGAC21890.10061559831348217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7800.0119.0443651
AGCGAAA17250.076.1172261
CAAAAGC81900.075.817784
GCAAAAG83800.073.9275363
GCGAAAG20350.064.4700552
CGAAAGC20750.064.251593
AGCAGGT97500.063.3955048
AAGCAGG114050.062.3893287
GCAGGTC62250.060.532429
TTTAAAC44500.058.617413
ACTATTC45000.057.487028
TAAACTA46150.057.4636885
GTAGAAA25800.055.3910981
GCAGGTA43100.055.1468439
CTATTCA47400.054.7304159
AAACTAT48700.053.7121286
AAAGCAG141450.050.45776
AGCAAAA128350.049.4711572
TAGAAAC28200.048.836932
GCGCGAA450.0096182748.3617741
Produced by FastQC (version 0.11.5)