FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2589_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2589_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839851
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6900.050.482871
GAGCAAA65550.044.172511
AGCGAAA12250.040.875671
CAAAAGC69700.040.2320334
GCGAAAG13500.037.052992
GCAAAAG76700.036.560913
CGAAAGC14100.036.487213
GTATACG1001.3843119E-436.284561
TATTCGC900.003612449432.2529451
AGCAAAA99800.027.748582
AAAAGCA104550.026.961395
AAAGCAG119500.026.8022386
GCTATTG15150.026.3081138
GAGCTAT17000.025.5752016
AAGCAGG134350.024.2720747
GTCCGAT1851.8200272E-423.535931
ATACCGG1550.00181467623.3751836
TAGTCGG3004.5811248E-721.7400688
CTACTGA31000.021.5051696
CGGAATA3106.2830077E-721.0376646
Produced by FastQC (version 0.11.5)