FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2589_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2589_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3480734
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10850.048.1423951
GAGCAAA78100.044.030361
CGAAAGC22450.037.765483
AGCGAAA22400.037.5694581
GCGAAAG22800.037.516512
CAAAAGC103750.032.5446554
GTTACGT5350.031.1888261
TATATCG2601.10958354E-1030.654995
TGACGCG2003.2644311E-728.993727145
GCAAAAG117500.028.6157823
GCGATTA2654.5765773E-927.352575145
ACATGGT24650.027.042875
AAGCAGG149650.026.4856827
AAAGCAG151550.025.722946
AGCAAAA135500.024.8229832
CCGACTA9450.024.5357884
AGTCGAA2751.9742947E-723.7238462
TAATCGT5850.023.535499
TTACGTG6600.023.064852
TACGTGT6650.022.8835373
Produced by FastQC (version 0.11.5)