FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2589_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2589_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172749
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA17500.054.3261031
CGAAAGC19650.048.6806643
GCGAAAG20500.045.974762
GTATTAA23500.025.9411
GTATATA18550.023.4737591
GTCGTAC4154.3655746E-1122.7337231
TACACCG2205.926827E-419.7630145
ATTATAT22050.019.7477661
CGTACTC5900.019.6522873
TATATAC19500.018.953123
CTAACCG13300.018.5256043
TATATTC29800.018.4817243
CTATGCT33000.018.2258914
GAGGTCG14700.017.7472179
TCTATGC32400.017.669623
CTATACC17450.017.4412844
GTATAGG25550.017.3266351
ACTCTAT41150.016.9307691
GAGCGAA11600.016.8921
CTTATAT12450.016.880053
Produced by FastQC (version 0.11.5)