FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2589_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2589_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088717
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC19500.042.352283
AGCGAAA19950.041.8176351
GAGCAAA72900.041.1981161
GCGAAAG20400.040.5054322
CAAAAGC87850.032.9856264
GCAAAAG98650.029.3744283
CGGTTAC1358.0576196E-426.8683361
GAGCGAA14050.026.332881
AAAGCAG128100.025.3370786
GCGTTAA3751.0913936E-1125.1174139
AGCAAAA116050.025.1084272
GTTACGT3902.0008883E-1124.1815031
AAGCAGG133650.023.2553547
AAAAGCA125600.023.1296085
GCTATAT8900.022.8229921
ACTCCGA5450.022.6285611
GTATATA14650.021.788111
GTGATCG4701.0913936E-1121.5820629
TATATAC19100.020.8610343
GTATAAG6300.020.7270011
Produced by FastQC (version 0.11.5)