FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2589_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2589_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3392119
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7950.053.8429951
GAGCAAA71850.046.953771
AGCGAAA16250.044.646861
CGAAAGC18400.040.564383
GCGAAAG18900.039.1199462
CAAAAGC89150.036.8188554
GCAAAAG99450.032.1335873
TATATCG2951.4551915E-1129.4753555
GCCGACC3251.8189894E-1228.995209145
TTATCGC1255.1618397E-428.9947852
GACGAAT2405.287984E-827.18301145
AAAGCAG146700.025.3886246
TATCGTG3501.3096724E-1024.8438787
AGCAAAA132550.024.3354932
GCGATTA2101.5584857E-524.162676145
CGTTTCG2701.6581726E-724.1562659
AAAAGCA137200.024.0829965
TGACGCG2151.8698318E-523.600754145
GCTATTG17200.023.59318
ACATGGT20250.023.2588445
Produced by FastQC (version 0.11.5)