FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2589_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2589_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3784907
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA13450.055.5576551
GAGCAAA91900.048.5499271
AGCGAAA26950.041.994731
GCGAAAG29550.038.5131762
CGAAAGC30050.038.3370743
CAAAAGC127250.034.9011964
GCAAAAG142500.030.9139863
AAAGCAG177350.029.2911936
AAGCAGG185500.027.8871027
AGCAAAA164450.026.9323652
TGACGCG2301.1068114E-625.211279145
ACTCCGA8350.024.327671
GCTATAT9250.024.3135831
CTATATA12000.024.1627242
AAAAGCA187150.024.1176995
ACATGGT31600.023.8442635
GTCGTCT4455.456968E-1222.803463145
GTATATA19100.022.7901331
TATATCG2603.227391E-622.2922675
CGTATGC2953.9052247E-722.1039358
Produced by FastQC (version 0.11.5)