FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2588_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2588_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3515767
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6700.069.298921
AGCGAAA13350.052.7122731
GCGAAAG15050.047.2024572
CGAAAGC15450.045.0195963
GAGCAAA81200.043.68921
TTACGTG5750.040.341982
CAAAAGC90150.037.8525774
GTTACGT6150.036.5685581
TACGTGT6600.035.1289253
CGAAGCG2009.0203685E-932.604039
GCAAAAG108750.031.6463153
AGACGAC4600.031.501483
GACGACT4650.029.6033554
GCTATTG14300.027.3596388
AGCAAAA128950.026.4211432
AAAGCAG156100.025.388146
AAAAGCA135850.025.385975
CCGACTA7750.024.3059124
GAGCTAT17200.023.1675826
AAGCAGG179700.022.3364587
Produced by FastQC (version 0.11.5)