FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2588_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2588_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3282106
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA11000.048.1445051
GAGCAAA74450.047.9420851
CGAAAGC20850.043.0958673
GCGAAAG21450.042.248172
AGCGAAA21250.041.308841
CAAAAGC106450.034.034384
GTTACGT4300.032.0554351
GCAAAAG112500.031.6907563
GCTATAT7000.031.0913621
AAAGCAG143850.029.065166
AAGCAGG148050.027.947387
ACTCCGA6900.027.3363671
AGCAAAA131100.026.7650382
TATAGCG1400.001000118825.8920922
GGTTATA8550.023.7579231
CGTATGC2751.9810432E-723.7153668
AAAAGCA156550.023.3276525
AGACGAC5050.022.958773
TTACGTG6000.022.9576572
CTATATA10500.022.7850422
Produced by FastQC (version 0.11.5)