FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2588_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2588_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3483481
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA94740.2719693318264116No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG74760.21461291162489476No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG66720.1915325503426027No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA64310.18461418334131866No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC55770.16009847620813777No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT52100.14956303766261392No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC47780.1371616495109346No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG46890.13460673389635253No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG44350.12731517697383737No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC41710.11973655088114447No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG36890.10589981687857633No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9350.077.600981
AGCGAAA16200.065.8386841
GCGAAAG19500.055.3859832
CGAAAGC20800.052.2413333
AAGGTCG7200.046.2846079
CAAAAGC88000.046.1793484
GCAAAAG91050.044.0774153
AGCAGGT61100.038.0600058
CTATTCA27200.035.956399
GTAGAAA30900.035.926241
CTATAAC37100.034.58182
AAGCAGG141850.033.9092987
ATTCAGC47700.033.7138566
ATTATAT55050.033.0822641
GCTATAA39650.032.389851
ACTATTC29850.032.2784238
CAGTACG13800.032.0239
TAACTAT39650.031.971465
TTATATT58300.030.4611112
GAGCAAA144350.030.2090111