FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2588_d6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2588_d6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3490869
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC19150.044.6506233
AGCGAAA19100.044.453071
GCGAAAG21050.040.9875372
GAGCGAA11450.039.294841
GAGCAAA96650.035.21441
CAGTACG5700.034.324429
CAAAAGC108900.030.2729154
GTTACGT4650.029.6517261
AAGCAGG129750.028.6487487
ACTCCGA7500.028.0598981
TATATCG1555.52742E-528.047255
GCAAAAG116550.027.7913443
TTACGTG5300.027.3596862
GTCGTAC2456.3892E-826.6578881
AGCAGGT42650.026.1639658
GCTACTA6700.025.9567769
ACATGGT27000.025.4935675
ATTATAT24450.024.6347711
AAAGCAG153650.024.2861046
CTATAAC17100.023.7437272
Produced by FastQC (version 0.11.5)