FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2588_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2588_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3061779
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA73860.24123230318060188No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG55130.1800587174972459No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG37910.12381690513913644No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG33820.11045865818532297No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8000.069.849521
AGCGAAA16100.051.8363841
AAGGTCG6900.051.45769
GCGAAAG18000.046.722082
CGAAAGC18800.045.8630873
CAAAAGC81700.037.7787484
CGACGAA1406.6284156E-736.241005145
GTCGTAC1905.3823896E-934.3757061
GCAAAAG88150.034.358023
TGACGCG1707.848939E-834.10918145
GCCGACC3100.032.73381145
GTAGAAA28000.032.6569181
GAGCAAA103700.031.7716521
AGCAGGT50400.031.3405958
CTATTCA23450.030.2820859
AGCAAAA103800.029.5447082
ATTATAT46500.028.8723071
ATTCAGC41200.028.6653656
AAGCAGG130050.028.5250667
GGTCGTT7000.027.9468487
Produced by FastQC (version 0.11.5)