FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2588_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2588_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543125
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA14250.043.7805021
CGAAAGC14900.042.788753
GTTACGT4200.041.45331
TACGTGT4400.041.164283
GCGAAAG15500.041.1510242
GAGCAAA64550.039.6712271
GAGCGAA7100.037.804241
TTACGTG4950.035.1427462
ACTCCGA4550.035.075871
ACATGGT23450.033.983335
ACGTGTT5450.031.9029484
GTCGTCG1753.7878744E-628.989914145
CAAAAGC88000.028.4845624
AAGCAGG96950.028.0984127
GGTCCTA37750.026.8711959
GACATGG30850.026.3014074
GCAAAAG95450.025.8828243
GATAGCG6350.025.109375145
ATTATAT17050.025.1029511
GCCGACC2901.196895E-824.991306145
Produced by FastQC (version 0.11.5)