FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2588_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2588_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364871
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9400.054.7909851
AGCGAAA15150.048.838931
GCGAAAG16600.044.9784162
CGAAAGC17950.041.9801333
GAGCAAA79200.041.582371
CAAAAGC86700.037.1885644
AGTCGAA2404.3655746E-1133.224352
GCAAAAG100250.031.4398023
CGACGAA1555.512464E-528.058363145
GTCGAAG2954.8748916E-1027.0175273
AGCAAAA118600.026.5265082
TATATCG2756.8212103E-926.3476725
GCTATAT11750.025.3118921
AAAGCAG146750.025.2300286
AAAAGCA130050.024.7927425
GAATCGC3352.1009328E-923.8191621
GTCGAAT1851.8394558E-423.4992733
TACCATG15200.022.8812127
AAGCAGG168350.022.595797
CTATATA16750.022.0714262
Produced by FastQC (version 0.11.5)