FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2588_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2588_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3788845
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA84450.042.000081
ACTCCGA7950.039.2321621
GAGCGAA11750.037.0385781
CGAAAGC19000.036.9933133
GCGAAAG19450.035.775262
AGCGAAA20000.034.453591
ACATGGT34400.031.5951715
GTCGGCT10100.030.8485666
AGTCGGC10100.030.1311575
GCTATAT7250.030.014021
AAGCAGG143850.028.0064837
CAAAAGC127900.027.9862234
TCGGCTG11200.027.819167
GCTACTA9550.025.0385939
GCAAAAG142200.024.663283
AAAGCAG167000.024.0804396
GGTCCTA60550.024.0536659
GTTGACA48100.023.6752991
TATTGGG19350.023.5988462
GACATGG49100.023.1689344
Produced by FastQC (version 0.11.5)