FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2588_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2588_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4249564
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG15650.045.850342
AGCGAAA15350.045.845781
CGAAAGC16150.043.9675373
GAGCGAA9050.039.2810861
GAGCAAA79100.029.9921281
CAAAAGC93500.025.4164714
GCAAAAG106550.021.5568073
GTATATA38000.018.7102031
TTACGTG8650.018.434372
AGCAAAA135050.017.281562
TAGTGCG2952.1377037E-417.1929159
AAAGCAG165050.016.6370666
AAAAGCA149900.016.191655
CCGACTA8100.016.1005444
TATACTG27450.016.1003535
GATAGCG7503.092282E-1115.462163145
AAGCAGG175100.015.4340847
GTATTAT55050.015.4192951
TTATACT26200.015.2094834
GTTACGT10050.015.1596511
Produced by FastQC (version 0.11.5)