FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2588_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2588_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3547430
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC18950.039.387853
GCGAAAG19950.037.7932782
AGCGAAA21800.033.614921
TATATCG2206.2755134E-1032.93775
GAGCAAA80650.030.767271
GAGCGAA14900.028.7298241
ACGCGCC5900.028.2486618
CAAAAGC87650.026.9510384
GTTACGT7050.024.6995681
GCAAAAG97500.024.1553343
GTATTAT34700.023.418321
AACGCGC4801.4551915E-1121.1350237
CGCGCCA7250.020.9907449
TTACGTG8300.020.9631842
AAAGCAG131650.020.9160026
TACGTGT8050.020.7045733
AAGCAGG131750.019.965127
AGCAAAA121150.019.5680922
GTATATA21750.019.3479941
TATAATA24400.019.3129062
Produced by FastQC (version 0.11.5)