FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2588_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2588_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2965060
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA17950.050.110881
GCGAAAG18550.048.8382152
CGAAAGC22200.042.0973363
GAGCAAA54450.040.7660261
GCAAAAG61150.034.5942763
CAAAAGC61700.034.5207334
GAGCGAA11450.031.6766571
AGCAAAA76500.027.4745252
CCGACGC5050.024.401125145
AAAGCAG119450.021.4102176
AAAAGCA104700.020.8275265
GTATATA22550.019.3009871
GCTATAT12100.017.3855111
TATATAC24650.015.870653
GTATAGA20950.015.5812861
GTAGAAA31250.015.5524781
GTATTAT22500.015.4751021
GAAAGCA64350.015.1985834
AAGCAGG175050.014.7339917
TACCATG17750.014.6938867
Produced by FastQC (version 0.11.5)