FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2588_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2588_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3677067
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG23400.048.3187752
CGAAAGC24650.047.0267373
AGCGAAA26750.042.0269971
GAGCAAA89200.040.8187141
CAAAAGC93350.038.2610324
GCAAAAG93950.037.0157243
AGCAAAA115150.030.5900022
AAAGCAG155500.026.3710756
AAAAGCA142150.025.4321845
GTGATCG7700.025.4071649
GAGCGAA24150.023.1256541
GTATATA27500.020.8360231
AAGCAGG185500.020.8176177
TAGTACC14800.019.5804354
TTATCGC3401.5214537E-619.1853962
CCGACGC5209.2040864E-1018.120035145
ATATATC28300.017.6646273
TATTATA31300.017.1354122
TATATAC28550.017.002413
TTAGAGT21850.016.2468324
Produced by FastQC (version 0.11.5)