FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34901971
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6806731.9502422943391935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC161150.037.520523
GAGCGAA89950.035.3585851
GAGCAAA598500.032.2252541
GCAAAAG611600.031.83993
CAAAAGC774950.025.5495364
GCGAAAG254100.024.043892
AGCAAAA825100.023.4626222
AAAGCAG1084750.021.146726
GCAGGTA231600.019.9691079
AGCGAAA318650.019.3685261
AAGCAGG1200550.018.8807727
CTATGCT172050.016.8975874
AAAAGCA1233650.016.566785
ACGGATA66950.016.2432823
CAGTACG55900.015.8206589
TCTATGC194950.014.8382533
GCTATAA164700.014.4500321
TATATTC345200.013.8193693
AGCAGGT670200.013.6066978
GTATAGG162950.013.4886091
Produced by FastQC (version 0.11.5)