FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_Mirella_HA_sample_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_Mirella_HA_sample_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCACGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAAGATGTCTT10.9259259259259258No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG10.9259259259259258No Hit
GGGAACATCTCTCTCATTTCTGATGATGAGTCCTCTTTCTTTGAATCTCG10.9259259259259258No Hit
AACTTCACTACATCCAGAGCAGAAATGAAAAATGTCGAGAGCAATTGGGA10.9259259259259258No Hit
TTAGTTGTAAGGCTTGCTTAAAAGTATTTTGATGCAAACTAATCACTCTA10.9259259259259258No Hit
CTCCAGAGCAGAAATGAAAAATGCCGATAGCAAATAGGACAGAAATAGCC10.9259259259259258No Hit
TCCCAGGACCCTGGGATCATGACCTGAGGTGAAGGCACACAATCCACTCA10.9259259259259258No Hit
GCACTCAAATGCAAATGTTGCACCTAATCTTGCCCTTTTGGCAGGCCAAA10.9259259259259258No Hit
AATAAATGATAAATCTAATAACATAAATATAAATATCAAAATCTATAAAC10.9259259259259258No Hit
GAATGTGAGACCTTCACTACCTCAATCTCAGAAATGAAAAATGGCGATAG10.9259259259259258No Hit
GCAGAATGCAGTTCTCTTCACTGACTGGTAATGTGATGTGATCATTTATG10.9259259259259258No Hit
AATATACAGAGATTCGCTTGCACAAACTGTGAAGAGAATGGGAGACCTGC10.9259259259259258No Hit
CAGTATCTGAAACACTACTGGAGCTGGCAAACAGAATTTTCGACCGTATG10.9259259259259258No Hit
AGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAACAGAATCTCT10.9259259259259258No Hit
GTAGTAAACAGTATCTGCAACACTACTGGAGCTGGAAAACCGAAGTTTCT10.9259259259259258No Hit
GCACCACCAAGTACAAATGCCAAGTCATTGGTTACATCCCCCCTTCTAGT10.9259259259259258No Hit
GTATACTAGAGTCCCGTTTTCGTTTCAATACCAACACCACGTCACCATGC10.9259259259259258No Hit
ATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACATTCACT10.9259259259259258No Hit
AATACAATCTGGTTAACAAGCAATTGTTCAAAACATACTCAAGCTCAAAT10.9259259259259258No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAATTGAGAAGCCA10.9259259259259258No Hit
CATATAAACAAAGAGGTGGTTCTTAGAAAGGCTGAGAGGAGCCATTGTCT10.9259259259259258No Hit
GGTGAGAACGTAGCAAGGCGCCAACGACAAAGTTCTGAAGTATTATCCCA10.9259259259259258No Hit
CAATGAGGCTGCAACATTGTCTTTAAAGTATATTTGTTTCCTTTTTCACA10.9259259259259258No Hit
GCTCGGGAGTTAGTGAAGGACTCATCAATACAAGACAGTAACTCCCAACG10.9259259259259258No Hit
CTTATGAGGATGTCAAAAATGCAGTTGTGGTCCTCATCGGAGGACTTGAA10.9259259259259258No Hit
GACAAAAACCACAGTGGACCATATGGCCATAATTAAGAAGTACACATCGG10.9259259259259258No Hit
CTCAGAGCTGCTCGATTGTGAGACAATAATTTTGATGGCTGTCAAAATAA10.9259259259259258No Hit
ACATTTGAGGAAAGCAGGTGTATAAGTGAAGAAATGAGGCACATATTGAA10.9259259259259258No Hit
AATTTGCACAATAGCCAAAGCAGAAATTATCTCAAAAACAATACAAAACT10.9259259259259258No Hit
ATTCATGAATCTTGTAACAAAACCTTACTGCCCAGGGGTTTCTTTTTGCC10.9259259259259258No Hit
CCCTTGGCCTCGATATCGAAACAGCCACACTTTTACCGAAACAAAACGTG10.9259259259259258No Hit
ATCACAAGCTGTCAGGTAGACTGTTACCTATGGCAAATACGCAAGCGATA10.9259259259259258No Hit
TTTATCATTTCTGCTCTGGAGGTAGTGAAGGTCACACAATCTCATCACAG10.9259259259259258No Hit
GTATCTGCAACACTACTGGAGCTGGAAAACCGAAGTTTCTACCGGATTTG10.9259259259259258No Hit
GTCCTCAGTTAAAGAAAAAGACATGACCAAAGAGTTTTTTGAGAATAACT10.9259259259259258No Hit
TCCTCGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAAT10.9259259259259258No Hit
CTGTAACACAGTGGTATGTAGCAAACCATCAAGCTATGGCAAGCAAAATT10.9259259259259258No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC10.9259259259259258No Hit
ATAATGAAACAAATATAAATATATTAAGCCACAGCATTAGGCAACTTAAT10.9259259259259258No Hit
CCCAGGTTCGTACCTAAGAGCCTGAACAATAAGGAGCAGTTTTCTTGACT10.9259259259259258No Hit
CAGCAATAGTTGGAGAAATTTCAAAATTACCTTCTCTTACAGGACATTCT10.9259259259259258No Hit
GGTATGGGGAGGATCTGAAAATTGAAAATAACAAATTTGCAGCAATATGC10.9259259259259258No Hit
ATATAGCCACCCAAGATCAATGGTTCCAGGTTCCCGTGTTTCCCTCAGAG10.9259259259259258No Hit
CAAAAAATGACAATTACAACAGAACAAAATAACATCAAAATTTCTGACAA10.9259259259259258No Hit
ACTTAAGTAAAAAGAAGAAAGAAGTCCAAAATTTCACCAAGCATACAATG10.9259259259259258No Hit
GCCCTGTAACTGACTCCAATGCTGGGATCATTAGAATTCGCAGTCTAATC10.9259259259259258No Hit
GTATGTAAGAATGTATACGGTGGTGTGTATGTGTGCATATACACTATAAG10.9259259259259258No Hit
AAATATTAGTGCCATAACAGTATGATAATCGGAAAACTGGTGGGATTTGG10.9259259259259258No Hit
TGCTAGCCGTGTTCGAAAGGAAGAGGCCAAATATCGGTAGATCCGGCCCC10.9259259259259258No Hit
TCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCCT10.9259259259259258No Hit
CTATATTTACACACAATCCAAACTACATACAATTCCATTCAAGACCTACG10.9259259259259258No Hit
GAACAAAAGTATGTTAGATTATAAAAACAACAGTAGGTAACATTTGTAGA10.9259259259259258No Hit
GTATACATTGTGGTCATAAGTTCCATTCATGATTGACCACATGCATGCAT10.9259259259259258No Hit
ATTAACCACCTGATGTCCTCAAATTACTGTCCCAATTGCTCTCACCCTTT10.9259259259259258No Hit
GTAGAATCAAGGGTCTTTAATAACATTAAATAAGCAGAAACGAGAAAGCT10.9259259259259258No Hit
GCAGTATATACAATGAAGTTTTACAGTTGCCTCAAGGAACATGTGGTGAC10.9259259259259258No Hit
AACCAAGACGCATTGAGCAAAACCCAGGGATAATTAATCAGGCCCTCCTC10.9259259259259258No Hit
CCCCAGGACCCTAGGATCATGGCCTGAGACGAAGGCAGACTCTTTAACCC10.9259259259259258No Hit
TTCCTATACAGTATAACTGCTTTGTCCATGGTATTTCAGTCTCCTTTCCC10.9259259259259258No Hit
GTTAAAAGTCAATATTACAATTGGCTAATTAAACTTGAACATGAAGTACA10.9259259259259258No Hit
GTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGCA10.9259259259259258No Hit
CAATAGTAACACCAGCAGACAAACTTTTGAGGTAGACATAAGTGAATCTA10.9259259259259258No Hit
AAAATTAATACAATGAAACAAAATTCACAGAATGCAGTAAAACAGGTTCT10.9259259259259258No Hit
TTCTAAGTGTCTCGATGGATTCCTCTTTAAAGATCCATTCCACGAATTGT10.9259259259259258No Hit
TTGGTTTGCTTCTCCCCACAACTGAGACCGTTTAATTCCCACATGCCTAG10.9259259259259258No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGTCCCTAAGCGC10.9259259259259258No Hit
GGGTTTATCTTTCTCTCCTCAACATTCAGCCAAACTGTACAGTTTGTCAT10.9259259259259258No Hit
ACAAACAAGAGTACAAAACATATAGAAAGTACTAAAATTAAACCAACAAA10.9259259259259258No Hit
AGACAGAAATACTCCATTGTACAATTGAGTATAATTTCTGTTTAATTCTT10.9259259259259258No Hit
GTTTATAGTTTTACATTCCTAAATCTAAAATCTGGATACTTATCTATTTA10.9259259259259258No Hit
CTCTTATCTCTTATACTACTTCAAGCACTAGTTCTAAGGCATGCATAAAT10.9259259259259258No Hit
TCAATAAATAATCTGAAAAGAGAAATCACTAATATCTACTAAACCTTCAA10.9259259259259258No Hit
GTCCGAAGGTGGTGGAGAGGATGGAGGGGACAGAGGACAGAGCAAAATCC10.9259259259259258No Hit
CAGTATCTGCAACACTACTTGAGCTCGAAAACCGAAGTTTCTACCCTAAT10.9259259259259258No Hit
GAGTCATTCTGTAAACAGTACAACATGTTGCCCTCTCTCTGGTGTGGAAA10.9259259259259258No Hit
CAATTCTTGGAAAAGATGCCGGAACTTTAATTGAAGACCCAGATGAAAGC10.9259259259259258No Hit
CTCTTATAAACAGATTGAAACTGATGGAGATAACCAAAATGCAAATTAGA10.9259259259259258No Hit
AAAAAGATACAGATAATAGCTTCCAGAATCTGTCATGAGAATGGGAGACC10.9259259259259258No Hit
ATTTTTCATTTCTGCTATGGAGGTACTCAACCTCTCCCCATCTCATCAGA10.9259259259259258No Hit
ACTAACGAGAGCAATAGGCCAAATTTCAAGGCCGAAGATCTTGTATGACA10.9259259259259258No Hit
AGCAATATGAATTCAACTCCCCCCCAAACAACAAAGTGATGCTGTGTGAA10.9259259259259258No Hit
CCAAAAAACATCAAAATAATTAAAAAATTAATAAATCAAAAAATCAAATC10.9259259259259258No Hit
CATCCACAGAATTATGCTGTTCCTTTCTATATTCTTCCCTCATAGAATCA10.9259259259259258No Hit
CTCCACCACGATGTCAATCTTTCAGGTTGCCTGTGTCCGATGGCATATCC10.9259259259259258No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGG10.9259259259259258No Hit
CAACACTATCTACAACCCTCCCATTGTAAATCAAACTAGCAGTTGCATTT10.9259259259259258No Hit
GTACGGATAACAAATAGCAGCACTGCTATAACTAGTTTAATGCATGTGTC10.9259259259259258No Hit
GAAGAGAAGGTAATGGTGAACTTTCTCCAACTATTGATCCCTCCTCAGTT10.9259259259259258No Hit
ATACAGAGATTCGCTTCGACAAACTCTGAAGAGAATGGGAGACCTTCACT10.9259259259259258No Hit
AAAGCAGGGTGACAAAAACATAATTGACTACAACACCATGTCAAGCTTTC10.9259259259259258No Hit
GGGGAGGGTAGGTGTTATAATGAGCCCTGGGTATTATATAAGACTAATGA10.9259259259259258No Hit
CCTACAAACTACTAACATATTACAATTACCAGTGAGCAAAATTAGGAAAA10.9259259259259258No Hit
ATTCTATTAACCATGAAGACTATCATTGCTTTGAGCTGCATTCTATGTCT10.9259259259259258No Hit
GGGTTATGAGTTCGAGCCCCACCCTGGGGGGATCGATTCGTTTATATAAT10.9259259259259258No Hit
TACGAAAGCAGGGTGACAAAAACATAAATTACTCAAAAAAAATGTCAAGA10.9259259259259258No Hit
GCTCCAGAGCGTACAACCCCTCAAGTGATAATATCTCCTCAAGGAAGAGG10.9259259259259258No Hit
TCGCTAGTCCGGCCAGGCAGATGGAGCAGGCAATTCGAGCGATTGGGCCT10.9259259259259258No Hit
CCTATATGATGACAAAAAGACTGTAGGAAGCACACCCACCATTGGGCTCA10.9259259259259258No Hit
GTCCCGGTCATCCCCCCACGCATCCATTACCTCTACCTGCAGAACAACTT10.9259259259259258No Hit
GTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTT10.9259259259259258No Hit
TTTTTAGCACATAGACTCATGTACACCTTCCGGAGAAGCTCCAGTTTATA10.9259259259259258No Hit
AAAATTATCAGTATCTCAAAAATGCAGTTAGGGTCATAAACCGGGTACAA10.9259259259259258No Hit
GATTTACTAAAGGATCTGATTTAATCAATTTATTATGTGGAAATTAATAT10.9259259259259258No Hit
CAAGACGGAACAGCATTAAGCATCAATGAACTGAGTAACCTTGCTAAGGG10.9259259259259258No Hit
CACTCACTCCGCCTGGGATCATGCCCCCACTCAGCCAGCGGACCCTTGTT10.9259259259259258No Hit
GAAAGCGAAAGAGAAGAGAATCGAAGAAATAAAAATAATTCGAGCCTTAC10.9259259259259258No Hit
CCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCAT10.9259259259259258No Hit
TGTGTGAACAGTTGCTCAATTTGCCAAGTACATCTGGGAATGCAGAACAC10.9259259259259258No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers