FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_Blank_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_Blank_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268589
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGATTCGAACCCC75782.8214111523554575No Hit
GGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTC54602.0328457233915014No Hit
GTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCC54192.0175807646627373No Hit
GGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGC53932.007900546932302No Hit
CCCCTGTACTCACCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGG45821.7059522169560182No Hit
GAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCCGCTC40961.5250066086101812No Hit
CCCCTAGGGGATTCGAACCCCTGTACTCACCGTGAAAGGGTGATGTCCTA39111.4561281362974656No Hit
GTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTT38601.4371400169031494No Hit
AAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGG32151.1969961539750325No Hit
CCCTAGGGGATTCGAACCCCTGTACTCACCGTGAAAGGGTGATGTCCTAG30421.1325854744609793No Hit
CGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGG29951.115086619332884No Hit
GTATTAAAGATGTAAGCAGTACGTTGTCCCCTTCGTCTAGAGGCCTAGGA28781.0715256395459232No Hit
AAAGCAGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTAC27931.0398787738887296No Hit
GTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGT25470.9482890215161456No Hit
CCTGTACTCACCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGGGA24890.9266946896559427No Hit
GTCCTAGGCCTCTAGACGAAGGGGACAACGTACTGCTTACATCTTTAATA23100.8600501137425584No Hit
GATGTAAGCAGTACGTTGTCCCCTTCGTCTAGAGGCCTAGGACATCACCC22450.8358495694164689No Hit
AGCGAAAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACG21930.8164891339555976No Hit
GGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACG21480.7997349109606127No Hit
GTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGA21070.7844699522318487No Hit
ATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCCGCTCCG20900.7781405791004099No Hit
GCTTACATCTTTAATACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTT18610.6928801998592645No Hit
GCGAAAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGC18280.6805937696629423No Hit
ACGTACTGCTTACATCTTTAATACAAAAGCCCGCTCAAAAACGTCTTGAA17910.6668180752003992No Hit
TTCGAGCCCCGTCCGCTCCGCCAGAACGAAGCCCGTTCAAGACGTTTTTG16680.6210231990141071No Hit
GTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCT16220.6038966599525669No Hit
GTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCCGCTCCGCCAGAACGAA16000.5957057064883521No Hit
AGCGAAAGCAGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTT15690.5841639084251403No Hit
ATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAA14990.5581017837662748No Hit
GAATCTGGCGTCCCCTAGGGGATTCGAACCCCTGTACTCACCGTGAAAGG14620.5443260893037317No Hit
GCAAAAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGC13640.5078391147813202No Hit
GTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGGGACAACGTACTGCT13460.5011374255833262No Hit
GTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCC13290.4948080524518874No Hit
AGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGG13190.4910848917863353No Hit
CCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGGGACAACGTACTG13190.4910848917863353No Hit
GAACGAAGCCCGTTCAAGACGTTTTTGAGCGGGCTTTTGTATTAAAGATG12650.47097982419235335No Hit
GTACTCACCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGGGACAA12490.46502276712746987No Hit
GAGCAAAAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCAC12210.4545979172639237No Hit
CTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGATTCGAACCCCTGT11870.4419391710010462No Hit
CCTCTAGACGAAGGGGACAACGTACTGCTTACATCTTTAATACAAAAGCC11670.43449284966994184No Hit
CTTTAATACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTTCGTTCTGG11600.4318866372040553No Hit
GGTTCGAGCCCCGTCCGCTCCGCCAGAACGAAGCCCGTTCAAGACGTTTT11310.4210894712739539No Hit
GCGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGG11260.41922789094117774No Hit
GAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGC11220.4177386266749569No Hit
TCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCTG11030.41066462141040777No Hit
GCAGTACGTTGTCCCCTTCGTCTAGAGGCCTAGGACATCACCCTTTCACG10890.4054521964786346No Hit
CGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCG10620.39539966268164367No Hit
AGCCCCGTCCGCTCCGCCAGAACGAAGCCCGTTCAAGACGTTTTTGAGCG10340.38497481281809753No Hit
TCCTAGGCCTCTAGACGAAGGGGACAACGTACTGCTTACATCTTTAATAC10230.3808793360859901No Hit
CAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGT9920.3693375380227783No Hit
GTTCAAGACGTTTTTGAGCGGGCTTTTGTATTAAAGATGTAAGCAGTACG9710.36151890062511866No Hit
TTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGATT9620.35816805602612173No Hit
ATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTG9400.34997710256190684No Hit
GTCCAGAAGCTTACCAGGGAAGGGAAGAAAAAGCATGGTTTCAGTGCCAT9120.3395522526983607No Hit
TTACAGAACTTCCCGCTGGGAGCTGTCTGTGGTTCCAGTTAGCTTACCCC9050.33694604023247415No Hit
ATTCGAACCCCTGTACTCACCGTGAAAGGGTGATGTCCTAGGCCTCTAGA8670.3227980297033758No Hit
AACGAAGCCCGTTCAAGACGTTTTTGAGCGGGCTTTTGTATTAAAGATGT8660.32242571363682054No Hit
GGCTTTTGTATTAAAGATGTAAGCAGTACGTTGTCCCCTTCGTCTAGAGG8550.3183302369047132No Hit
CGCCGGGGGTCGCGGGTTCGAGCCCCGTCCGCTCCGCCAGAACGAAGCCC8390.3123731798398296No Hit
CATCATATGATTAAATAGAGGTAATAAAGGCCATCAGAATTCCAGAATGG8350.3108839155736088No Hit
GTGTTACTCTGTCCAGAAGCTTACCAGGGAAGGGAAGAAAAAGCATGGTT8330.3101392834404983No Hit
AAGCAGTACGTTGTCCCCTTCGTCTAGAGGCCTAGGACATCACCCTTTCA8180.30455454244217No Hit
CTTTTGTATTAAAGATGTAAGCAGTACGTTGTCCCCTTCGTCTAGAGGCC8180.30455454244217No Hit
GAAAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCG7870.2930127443789582No Hit
GCAGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAA7800.29040653191307164No Hit
ATTAAATAGAGGTAATAAAGGCCATCAGAATTCCAGAATGGGAGAACTTA7780.2896618997799612No Hit
GTTTTTGAGCGGGCTTTTGTATTAAAGATGTAAGCAGTACGTTGTCCCCT7630.2840771587816329No Hit
GATTAAAACAGAGAGCAATTGCTCTGGAAAATAAAGGTTGGCAGTTATTA7550.28109863024919113No Hit
AGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAAC7520.27998168204952545No Hit
GGTGATGTCCTAGGCCTCTAGACGAAGGGGACAACGTACTGCTTACATCT7380.2747692571177524No Hit
CCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGTCCC7340.27327999285153154No Hit
ACGTTGTCCCCTTCGTCTAGAGGCCTAGGACATCACCCTTTCACGGTGAG7310.27216304465186586No Hit
CCGCTGAATCTGGCGTCCCCTAGGGGATTCGAACCCCTGTACTCACCGTG7190.2676952518532032No Hit
CCGCCAGAACGAAGCCCGTTCAAGACGTTTTTGAGCGGGCTTTTGTATTA7120.26508903938731665No Hit
GTCTAGAGGCCTAGGACATCACCCTTTCACGGTGAGTACAGGGGTTCGAA7070.2632274590545406No Hit
TTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCCG6990.2602489305220988No Hit
GTTCGAGCCCCGTCCGCTCCGCCAGAACGAAGCCCGTTCAAGACGTTTTT6710.2498240806585527No Hit
AGCAAAAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACG6560.24423933966022435No Hit
GTACTGCTTACATCTTTAATACAAAAGCCCGCTCAAAAACGTCTTGAACG6480.2412608111277826No Hit
CAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCC6090.22674048453212903No Hit
TAGTTGGACTGATGAGATTAAAACAGAGAGCAATTGCTCTGGAAAATAAA5810.21631563466858283No Hit
TCCCAGGACTCTGGGATCATGACCTGAGCTGAGGGCAGACGCTTAACAAC5780.2151986864689172No Hit
GCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGG5650.2103585776036993No Hit
GCGAAAGCAGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTC5630.20961394547058887No Hit
GGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCCGCTCCGCCAGA5420.20179530807292928No Hit
AATCTGGCGTCCCCTAGGGGATTCGAACCCCTGTACTCACCGTGAAAGGG5320.19807214740737705No Hit
CACCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGGGACAACGTAC5320.19807214740737705No Hit
ACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTTCGTTCTGGCGGAGCG5310.19769983134082184No Hit
GCCTAGGACATCACCCTTTCACGGTGAGTACAGGGGTTCGAATCCCCTAG5300.19732751527426662No Hit
CATATGATGTGTTCAATTAAATCTTACCAATGTTGAAAGGCACCATAGTG5220.19434898674182485No Hit
CTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCCGCTCCGCCAGAACG5020.18690266541072048No Hit
TTCAAGACGTTTTTGAGCGGGCTTTTGTATTAAAGATGTAAGCAGTACGT5020.18690266541072048No Hit
CACTTCTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGATTCGAACC4980.18541340114449958No Hit
GGACAACGTACTGCTTACATCTTTAATACAAAAGCCCGCTCAAAAACGTC4970.18504108507794437No Hit
GCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGG4970.18504108507794437No Hit
AGCAGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGA4950.18429645294483393No Hit
TGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGTCCCCT4800.17871171194650562No Hit
GTGCCATACATTACAGAACTTCCCGCTGGGAGCTGTCTGTGGTTCCAGTT4770.17759476374683997No Hit
GTACGTTGTCCCCTTCGTCTAGAGGCCTAGGACATCACCCTTTCACGGTG4740.17647781554717432No Hit
GAGCGAAAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCAC4670.17387160308128777No Hit
TCATATGATTAAATAGAGGTAATAAAGGCCATCAGAATTCCAGAATGGGA4630.1723823388150669No Hit
GGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGCGCCCCGTC4590.170893074548846No Hit
CGTCCGCTCCGCCAGAACGAAGCCCGTTCAAGACGTTTTTGAGCGGGCTT4540.16903149421606992No Hit
ATACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTTCGTTCTGGCGGAG4540.16903149421606992No Hit
ACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGATTCGAACCCCTGTAC4420.16456370141740725No Hit
GCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGC4390.1634467532177416No Hit
GTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGCGCCCCGTCC4380.16307443715118639No Hit
GATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCT4120.15339421942075065No Hit
AGACGAAGGGGACAACGTACTGCTTACATCTTTAATACAAAAGCCCGCTC4090.152277271221085No Hit
TTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGTC4080.15190495515452979No Hit
ACATTACAGAACTTCCCGCTGGGAGCTGTCTGTGGTTCCAGTTAGCTTAC4060.15116032302141935No Hit
GGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCG4050.15078800695486413No Hit
CTATTTAATCATATGATGTGTTCAATTAAATCTTACCAATGTTGAAAGGC4030.15004337482175367No Hit
CGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGA3990.1485541105555328No Hit
GGATAGCACTTGATCATACATGAGTCAAGGAGGAATGGGAGGAGTGGGAA3980.1481817944889776No Hit
GCCAGAACGAAGCCCGTTCAAGACGTTTTTGAGCGGGCTTTTGTATTAAA3910.14557558202309107No Hit
GCTGAATCTGGCGTCCCCTAGGGGATTCGAACCCCTGTACTCACCGTGAA3880.1444586338234254No Hit
GTGATGTCCTAGGCCTCTAGACGAAGGGGACAACGTACTGCTTACATCTT3870.14408631775687017No Hit
CGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATC3790.1411077892244284No Hit
CCATAGTGTTAAGTACATAGACACTGACATCAGATTATCTGAGTTTGAAT3540.13179988756054792No Hit
GGCGTCCCCTAGGGGATTCGAACCCCTGTACTCACCGTGAAAGGGTGATG3520.13105525542743746No Hit
TCTTTAATACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTTCGTTCTG3500.13031062329432702No Hit
CTGTACTCACCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGGGAC3460.12882135902810612No Hit
TTTTTGAGCGGGCTTTTGTATTAAAGATGTAAGCAGTACGTTGTCCCCTT3440.12807672689499572No Hit
GAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGCGCCCCGTCCGCTC3400.12658746262877482No Hit
GAATGGAAGAGGGAGAAGCAGGCTTCTTAATGAAGGCCTGATCCCAGGAC3340.1243535662294435No Hit
AGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCG3340.1243535662294435No Hit
GTCTATGTACTTAACACTATGGTGCCTTTCAACATTGGTAAGATTTAATT3330.12398125016288829No Hit
ATACATTACAGAACTTCCCGCTGGGAGCTGTCTGTGGTTCCAGTTAGCTT3310.12323661802977784No Hit
TCTTAATGAAGGCCTGATCCCAGGACTCTGGGATCATGACCTGAGCTGAG3290.12249198589666739No Hit
CTCTAGACGAAGGGGACAACGTACTGCTTACATCTTTAATACAAAAGCCC3290.12249198589666739No Hit
GACAACGTACTGCTTACATCTTTAATACAAAAGCCCGCTCAAAAACGTCT3290.12249198589666739No Hit
GGGGACAACGTACTGCTTACATCTTTAATACAAAAGCCCGCTCAAAAACG3260.12137503769700175No Hit
GCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCCGCTCCGCCAGAA3260.12137503769700175No Hit
AAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGAT3230.12025808949733607No Hit
GAGCGAAAGCAGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACT3140.1169072448983391No Hit
GCCCCGTCCGCTCCGCCAGAACGAAGCCCGTTCAAGACGTTTTTGAGCGG3110.11579029669867344No Hit
CGCCAGAACGAAGCCCGTTCAAGACGTTTTTGAGCGGGCTTTTGTATTAA3090.11504566456556299No Hit
ACGTTTTTGAGCGGGCTTTTGTATTAAAGATGTAAGCAGTACGTTGTCCC3070.11430103243245257No Hit
GTACATAGACACTGACATCAGATTATCTGAGTTTGAATCTCAGCTCTGTC3030.11281176816623167No Hit
ACTTAACACTATGGTGCCTTTCAACATTGGTAAGATTTAATTGAACACAT3010.11206713603312123No Hit
CTCAAAAACGTCTTGAACGGGCTTCGTTCTGGCGGAGCGGACGGGGCTCG2980.11095018783345559No Hit
GTAATAAAGGCCATCAGAATTCCAGAATGGGAGAACTTAGTTGGACTGAT2980.11095018783345559No Hit
GCACTTGATCATACATGAGTCAAGGAGGAATGGGAGGAGTGGGAACCAGA2950.10983323963378992No Hit
CGTTTTTGAGCGGGCTTTTGTATTAAAGATGTAAGCAGTACGTTGTCCCC2920.10871629143412426No Hit
GGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGG2880.10722702716790339No Hit
GCGACACTTCTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGATTCG2870.10685471110134816No Hit
TCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAAT2860.10648239503479294No Hit
GTACAGGGGTTCGAATCCCCTAGGGGACGCCAGATTCAGCGGCGTTTCGT2750.10238691830268551No Hit
TCCCCTAGGGGATTCGAACCCCTGTACTCACCGTGAAAGGGTGATGTCCT2730.10164228616957507No Hit
GATTTAATTGAACACATCATATGATTAAATAGAGGTAATAAAGGCCATCA2720.10126997010301984No Hit
AAAGCAGGGAGAATGGAAGAGGGAGAAGCAGGCTTCTTAATGAAGGCCTG2710.10089765403646464No Hit
ATCATACATGAGTCAAGGAGGAATGGGAGGAGTGGGAACCAGATGGCAGC2710.10089765403646464No Hit
GTTTCAGTGCCATACATTACAGAACTTCCCGCTGGGAGCTGTCTGTGGTT2690.1001530219033542No Hit
AAATAGAGGTAATAAAGGCCATCAGAATTCCAGAATGGGAGAACTTAGTT2690.1001530219033542No Hit
GTTGTCCCCTTCGTCTAGAGGCCTAGGACATCACCCTTTCACGGTGAGTA2690.1001530219033542No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGC100.0070844023145.024583
CGACATA100.007088348144.99759145
CTGGGAC100.007088348144.99759145
GAGCAAA1850.0141.1051
GAGCGAA1000.0137.773361
AGCGAAA6150.0121.443351
AGGACAA1250.0115.99806145
AGCAAAA2650.098.507272
ATCCCCA554.1472958E-1092.271194145
TTACCGG259.372903E-487.014753
GGACATA1400.077.67728145
GCGAAAG9900.075.442082
CGAAAGC10150.072.869493
ATAGCAC1100.065.9202653
GATAGCA1100.065.9202652
GCAAAAG4300.062.39433
GAGTACC350.00356389962.141823145
GAAAGCA12000.061.635454
GGATAGC1200.060.4269071
CAGGTCT753.7463906E-758.0098344
Produced by FastQC (version 0.11.5)