FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2606_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2606_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3790840
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG15350.048.6537132
CGAAAGC17600.042.4337773
AGCGAAA18000.041.0879821
GTATAAG11900.018.8886931
GAGCGAA11250.018.6910041
TATATAC37250.016.7401493
TATATCG5252.0198058E-816.5733055
GTATAGA27100.016.3210351
GTATATA32650.016.2116361
CACGAGC10800.016.11081145
ACACCGT10200.015.6390256
GTATTAA42850.015.3984751
TATAGCG3304.994942E-415.3805282
AGTACGG3304.994942E-415.3805285
GTGTCGA3808.417567E-515.2628729
TATAGTA18350.015.0153232
TACACCG6801.9863364E-914.928165
GTATAAT30500.014.5016411
TATACAT52150.014.3208915
TAGCGAT3100.005844275514.0338464
Produced by FastQC (version 0.11.5)