FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2606_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2606_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394874
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG1900.057.243792
CGAAAGC2100.051.7923
AGCGAAA2150.050.5875361
CTAGTAT1204.05076E-430.2120023
CAGGGCG1000.006070222228.9998479
TAGGACT2903.929017E-1027.5033384
ATATAGG1855.79898E-627.4357621
GCATAAT1606.8122135E-527.1908021
TAATACA4450.024.441174
CTATATC1550.001805284523.3899364
GGTTACT1550.001805284523.3899361
CATAATA2552.697352E-622.747862
TAGTACC1600.002172901822.656139
CTAGGAC2903.271798E-722.5027313
ATAGGCC1650.002596267721.9723644
ATAATAC3706.6102075E-921.5566713
GTATAGC1700.003087344821.3261171
ATAATAG2404.350221E-521.1484013
TTAATAC4151.0841177E-920.96643
TTTAATA5950.020.71682
Produced by FastQC (version 0.11.5)