FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2606_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2606_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608665
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6700.055.1957631
GCGAAAG7100.053.1074372
CGAAAGC7250.052.0086633
TATATCG1051.8524023E-434.529565
CTAGTAC2954.820322E-1027.03843
GAGCGAA5300.025.9948981
AAGGTCG2001.0646185E-525.3792255
ACCGTTC3452.9685907E-923.115488
ACGTTGT1650.002601842621.969268
ACACCGT5300.021.890446
GGTCGTT2104.316286E-420.7138717
GTATAGA10200.020.6161791
GTATTAA19600.019.9778161
AATACCG1850.005043326419.597865
CACCGTT4553.43789E-919.1204997
GTATAAG8350.019.1049791
TAGTACT6550.018.819934
GAGCAAA23900.018.8106651
TTATACT11250.017.4028994
TAGGACT9750.017.1054134
Produced by FastQC (version 0.11.5)