FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2606_d6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2606_d6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3193492
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG20100.051.581762
CGAAAGC21050.049.598273
AGCGAAA21650.045.8802341
TATATCG1956.9994712E-933.4629365
TACGAAC1855.8209043E-627.4327189
GCTATAT8600.026.9782941
GTATATA28200.026.4820181
TATATAC29800.020.9236953
TATACCA33750.020.1934385
ATATACG4102.1700544E-819.4520344
TACCATG23750.019.231487
AGACGGT10400.018.821724145
GTATAAT29450.017.972171
GTATAGG10700.017.6178341
GTATATC8850.017.2043781
ATATACC21550.017.158494
TATCGTG3854.9705395E-616.9479647
GTATAAA27900.016.8916511
TAGTACC12050.016.8471784
TCTATAC31300.016.6780133
Produced by FastQC (version 0.11.5)