FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2606_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2606_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3509638
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC13950.053.5343323
AGCGAAA14050.053.154061
GCGAAAG15700.047.1053122
TATATCG3201.8189894E-1229.4552545
GAGCGAA6650.029.4386371
AATACCG2601.1456541E-725.0979655
GTATATA32400.021.4833411
ATACCGC2455.1158506E-520.7157826
TATAATA35850.020.0223672
GTATAAT21300.017.3606571
TAGTACC13850.016.7506799
TATATAC35250.016.455093
GTCCTAT27800.016.4312781
CGCTAGG3554.7016347E-516.3392092
GTATAGA26800.016.2327461
GTATTAC14050.015.9978241
GATATCG4551.3743611E-615.9338559
TATACAT47100.015.7017715
ATTATAC24600.015.6210473
TATACCA42600.015.4882085
Produced by FastQC (version 0.11.5)