FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2606_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2606_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3546008
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC20000.053.293033
AGCGAAA20350.052.020141
GCGAAAG22450.047.4770932
GTGATCG6400.029.4528629
GAGCGAA9550.022.7772351
TATATAC25500.019.6196863
TATATCG3701.5709156E-719.596635
TATACTG18200.019.5212575
GTATAAT23050.018.8739781
TATAATA28450.017.5853062
TCTATAC26300.017.093033
TTATACT20700.016.813344
CTATAAG14250.016.7912161
ACTATAC13550.016.5884383
TATACAG27750.016.4611685
CTATACT9300.016.3726674
CTATACC27000.016.1127834
AAAGCAG109350.015.9138615
GTATATA23750.015.8753341
TATACTA10550.015.8073295
Produced by FastQC (version 0.11.5)