FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2606_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2606_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3346817
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA16600.059.4055631
GCGAAAG17100.058.515752
CGAAAGC19650.050.922113
CTACGCG1804.7043013E-628.194439145
GAGCGAA8650.024.309621
GCTATAT15150.023.9306071
TATATAC27800.023.4740243
TATATCG4501.3278623E-1020.9469435
TATACCA38200.019.550765
GTATATA24450.018.9800531
TATAGGT13850.018.3234864
GTATACG2050.00912369517.68531
ATTATAC21150.017.1415253
CTATATA22450.017.1178552
TTATACT18350.016.9911294
TATACTG18200.016.3343665
GTATTAA28050.016.2856081
ATATAGC13400.016.233283
CTAGTAC13050.016.1130333
ATATCGT7703.6379788E-1216.0084026
Produced by FastQC (version 0.11.5)