FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2606_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2606_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4114111
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA26250.057.177571
GCGAAAG28500.052.662912
CGAAAGC29500.051.123513
GAGCGAA14250.024.4236761
TATATAC27400.020.6406523
TATATCG4251.4715624E-920.4725385
GTATATA22500.019.9799231
TATACTG32050.018.5509095
CTATATA23600.017.8194922
TATTATA31100.017.7187292
GTATTAT31850.017.5293561
TAGTACC19100.017.4623954
TATACCA37500.017.2083055
GTATTAC15700.016.1641561
AAAGCAG151550.016.0754345
GTATTAG12500.015.6616811
ATAGTAC27900.015.5928823
GTATAAA26450.015.3513651
GTGATCG10450.015.2629429
CATACGC6251.0515578E-815.0814353
Produced by FastQC (version 0.11.5)