FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2606_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2606_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018386
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC4650.070.169753
AGCGAAA5950.054.8385471
GCGAAAG6000.053.173082
TGATCCG2850.035.616589
GACCGTC1153.161725E-431.5224467
ATATTCG1050.00770582827.6210218
GCACGAC2451.9093186E-623.6774866
AGACCGT1750.003655179920.71786
GTCATAG5204.3655746E-1119.5215842
AAAGCAG32250.019.110835
TTATACT6900.018.9153254
TATTATA11600.018.7522622
ACCGTCT2000.00791291218.1262978
TCTCCGC4959.758878E-917.575289145
CGACTGG3804.3817017E-617.1714387
AAGCAGG38700.017.0506976
TGCGAGA2700.002331843616.110682145
CACGAGC3153.506879E-416.11068145
AGTGATC7302.0008883E-1115.8907677
CCTAGAC5052.3387838E-715.7939833
Produced by FastQC (version 0.11.5)