FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2606_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2606_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2561678
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA49090.1916322035790603No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG43830.17109878759157085No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC16500.048.3395583
AGCGAAA18000.044.7132231
GCGAAAG17850.044.6836282
GAGCGAA9750.035.696211
GAGCAAA64200.027.7834111
ATTATAT28000.027.1904721
GGTCGTT3850.026.3639457
GCTATAA14500.026.0028421
CAAAAGC65050.025.971834
GGATCGC2001.0665424E-525.374306145
CTATAAC12950.024.636382
GCAAAAG70100.023.9973853
TATTAAT30700.023.382492
TTATATT33200.022.932172
GTATTAA34300.022.8304841
TAACTAT14300.022.3105685
ATTCAGC31750.022.152466
GTACGGA4607.2759576E-1222.0676291
AAGGTCG6250.022.0398279
TATACTA24800.021.635852
Produced by FastQC (version 0.11.5)