FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2606_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2606_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3448062
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA14300.043.1012231
GCGAAAG14950.042.19672
CGAAAGC14950.041.711683
GAGCGAA7900.031.2074681
GAGCAAA64800.026.9680021
CAAAAGC68350.024.6121714
GCAAAAG73150.022.9971523
GTATATA22750.021.992491
TAGTACT13650.021.2484744
TATATCG1800.00430559120.1417855
AGTACGG3259.830474E-720.0798075
TATACTG22900.019.9482825
ACACCGT9250.019.597416
GTATAAG14100.019.5420991
TTATACT23200.019.3777854
ACCGTTC7300.018.8695648
CACCGTT7900.017.4364347
CCGTTCA8400.017.2616319
AGCAAAA103600.016.3078462
GTATTAG22600.015.7215131
Produced by FastQC (version 0.11.5)