FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2606_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2606_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3822003
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC21350.041.7716643
GCGAAAG22500.040.6034132
AGCGAAA23950.038.447911
GAGCAAA83500.032.823121
CAAAAGC89350.029.7003154
GCAAAAG98750.026.7997233
GTGATCG5900.025.8048929
AGCAAAA119300.022.365672
GTATATA21250.022.1783351
GAGCGAA17000.021.7518311
AAAGCAG153600.019.873096
TATACTG23850.018.2405265
TATATAC22350.018.1670743
AAAAGCA147750.018.059055
GTATTAC12850.018.0559921
GATAGCG5651.4370016E-1017.964231145
TATAGCG2851.6334857E-417.8085162
AAGCAGG170150.017.6825647
GTATTAT29600.017.1467131
GTATAAT19650.016.9734361
Produced by FastQC (version 0.11.5)