FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2603_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2603_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3485381
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA13650.052.585841
GCGAAAG16700.045.1513372
TATACCG2900.040.0020265
CGAAAGC21200.035.567333
CGCGCCA5950.028.0254239
ACGCGCC6550.027.6719658
GGACCGT5250.027.6204476
TAAGACG4400.024.7171634
GAGCGAA9750.023.052781
GACGCGC5151.8189894E-1221.1163627
GTATATA35800.020.2527121
TGATCCG5900.019.6611679
AGACGCG5555.456968E-1219.5955876
AAGACGC6603.6379788E-1217.5766495
GTATAGG14950.017.459331
ACTAGCG3804.386671E-617.1719235
ATACCGT4659.1660695E-817.1514076
AACGCGC3854.970874E-616.9479397
CTAGCGT3905.617241E-616.7316176
GTATAAG22000.016.4783441
Produced by FastQC (version 0.11.5)