FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2603_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2603_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2878617
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA20200.054.1988641
CGAAAGC21050.053.043633
GCGAAAG21150.052.792832
TAAGACG10650.036.0820244
ACGCGCC11700.034.6999668
CGCGCCA12850.031.0303339
GACGCGC10250.030.4138557
AGACGCG10300.030.2688436
AAGACGC12150.025.0632635
ATAAGAC20150.024.1081753
GTATTAA27300.022.0434741
TATAGCG2404.3644824E-521.1471272
GTATATA21250.020.4718421
GAGCGAA12550.020.2203621
TACCGTA7750.018.7091968
TGATCCG10600.018.4665099
AACGCGC5158.058123E-1018.3005037
GGATAAG28600.017.9993531
AAAGCAG106650.017.8116865
AAACGCG7450.017.517856
Produced by FastQC (version 0.11.5)