FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2603_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2603_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3929607
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC15000.065.253143
GCGAAAG18450.052.6590352
AGCGAAA20200.046.3040961
TACGGAT9650.026.296942
ACGGATA10250.024.7572943
ATGCGTC10400.023.701557145
GTACGGA11750.022.8320521
CGGATAA12000.021.1468524
GTCGTAC7500.020.3020131
AGGTCCG30650.018.4497268
CGTACTC8450.018.018623
CTGATCG6503.6379788E-1217.8456529
CTATAAC28150.017.7719542
TCGTACT8600.017.7045672
GGTCCGG31800.017.5545399
GTATAGG16050.017.1668131
GTATATA22550.016.7200621
AGGTACT17800.016.7002454
GTACTGA23450.016.6959026
GTATTAA35050.016.5494291
Produced by FastQC (version 0.11.5)