FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2603_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2603_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4160342
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA25100.062.9716261
CGAAAGC28750.055.4813423
GCGAAAG29600.053.643182
GTGATCG6700.024.8873469
GTACGGA1850.00504788619.5956822
GTATATA27850.019.2649841
AAAGCAG135350.018.1061365
GTATAAA21750.017.6676481
GTATAAT28900.017.561531
TATATAC25600.017.559573
TATACTG27250.017.2947465
GAGCGAA16400.016.7997131
GAGCAAA62350.016.2799741
TATACAT32500.016.0626245
ATTATAC33500.015.7993843
GCAAAAG64250.015.6856963
ATAATAC35650.015.4566783
TATTATA30350.015.2891512
GTATAGT21000.015.1913191
GAAAGCA114050.015.1303514
Produced by FastQC (version 0.11.5)