FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2603_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2603_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256216
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC4650.043.659953
GCGAAAG5300.039.673482
AGCGAAA5450.038.5830881
TACACCG1209.559737E-636.2533535
GAGCGAA1902.074903E-730.5303551
TCTCGTT2451.9131785E-623.672832145
ACACCGT2908.245655E-620.001856
GGGTTGA8750.019.8875526
GTATATA12050.018.6539211
GTAAGGT4705.131369E-918.512353
TAGTACC7100.018.379799
TATACTA6003.3651304E-1016.9182325
GTATAAT10600.016.4172671
GTATAGG3554.6953537E-516.340191
CTATAGC4058.026305E-616.1132431
GTCCTAT9100.015.9361741
GTATTAA15500.015.4375271
CCTAGAC3355.596427E-415.1506563
GTATAGA12000.015.1061651
ATATAAC8851.8189894E-1214.7471273
Produced by FastQC (version 0.11.5)