FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2603_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2603_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2766367
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG17200.067.026422
AGCGAAA18700.060.87561
CGAAAGC20150.058.2931253
GACGTAT3800.036.253296
ACGTATG4550.033.460947
GAGCGAA7850.024.0153481
TAGTACC8000.023.5646364
TATGACG5500.022.4111233
GTATATA20000.021.3898261
CATATCG3301.1396623E-619.7723778
GAAAGCA63500.019.5253934
ATATCGC3502.002018E-618.6425259
GTATTAT17050.018.2864461
AAAGCAG98800.018.200035
TAATCCG2000.00791538318.1266425
GCCTATA7550.017.2866461
GCTATAT6755.456968E-1217.1870521
GTATAAG12250.016.573231
TAGTATA7451.8189894E-1216.5451222
TATATAC18800.016.1982773
Produced by FastQC (version 0.11.5)