FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2603_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2603_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3265123
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA13450.066.3117141
GCGAAAG15000.059.9429172
CGAAAGC17500.051.7943
CTACGCG1000.00607715528.999308145
ATACCGT2001.0651114E-525.3790596
CCTATAC8900.020.3684253
TCTCCGC14350.018.18772145
TAGTACC15200.018.12794
TAGTGCG2801.4275957E-418.1245679
GTATAAT12950.017.3579861
TATAATA25750.017.1775042
ACCGTCG3002.419421E-416.9162648
TATACGG2600.001810980316.7334465
GAGCGAT4801.3174395E-716.614187145
GTATACG6556.548362E-1116.605711
ATTACGC4058.032082E-616.1136883
GAGCGAA7651.8189894E-1216.1136881
GCGTTGT3605.303661E-516.1107259
AAAGCAG114900.015.7140025
TACCGTG3706.703533E-515.6753027
Produced by FastQC (version 0.11.5)