FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2603_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2603_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3513032
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG20650.062.1540222
AGCGAAA22450.056.524631
CGAAAGC24950.052.604643
GTATATA30100.028.1861291
ACCGTCG1500.001495190224.1661938
ATATTCG3001.7105776E-824.1661938
CTGATCG10600.019.1505689
ATAATAC35100.018.3864943
TCTATAC33300.017.4205463
GTATAAA25900.017.0783581
CCTATAC8600.016.8634953
TATACAG28100.016.7734765
TATATAC32150.016.4648553
AAAGCAG116300.016.4603945
GTATGAC15000.016.4362853
TATACCG3605.294617E-516.1140065
GTATAAG16200.016.1140041
CTATACC34750.016.0676354
GAAAGCA90300.015.9801694
ATCTATA24600.015.9174932
Produced by FastQC (version 0.11.5)