FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2603_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2603_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754881
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA8400.062.1470831
CGAAAGC8800.061.795733
GCGAAAG9050.058.486312
GAGCGAA3250.035.6947361
ACCGACG2050.00912699317.6836268
AAAGCAG64700.017.4823675
CAACGCG2500.001393860117.400199
TACAACG2550.001590612717.060474
TGTTCCG4307.6099786E-716.8606519
GAGCAAA26550.016.6583821
TTATACT10500.016.5730294
GACAACG4651.7221828E-615.5915697
TATACTG10300.015.4869325
TCTCCGC10850.015.368395145
GTATATA12450.015.1415921
CGATGTC2900.003754695415.0001657
TATATAC12500.014.5013993
CACGACG4001.3023632E-414.499746145
GTATTAG7053.3032848E-914.3981431
TAGACAG11600.014.3763885
Produced by FastQC (version 0.11.5)