FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n01_2603_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n01_2603_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4028721
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA25600.054.6638221
CGAAAGC26900.053.3691333
GCGAAAG30500.046.8321042
TATACGA3409.094947E-1125.587697
CCTATAC15900.019.608693
TACGAAC4908.627467E-917.7547259
GTATATA24850.017.506831
GTATAAT18900.016.8800411
GAGCGAA14850.016.1127681
TATACTG24750.015.8196125
GAGCAAA67650.015.6483191
GTCCTAT25950.015.6470811
AAAGCAG157550.015.5091775
CTATACT24300.014.91904454
CGGTGCG3901.050442E-414.8714779
TTATACT21050.014.8113154
GTATAAA24650.014.7073941
AAGCAGG169750.014.6508076
TCTATAC40100.014.2843333
TATAATA24000.014.19922
Produced by FastQC (version 0.11.5)